B16F10 세포에서 해조류 발효 추출물의 Tyrosinase 억제 효과

• Published in: Journal of the Korean Society of Integrative Medicine Vol. 13; no. 2; pp. 171 - 179
• Main Authors: 윤현서, 박충무, Hyunseo Yoon, Chungmu Park
• Format: Journal Article
• Language: Korean
• Published: 대한통합의학회 01.05.2025
• Subjects: 예방의학; Saccharina japonica; Undaria pinnatifida; whitening activity; tyrosinase; cytotoxicity; Hizikia fusiforme; Sargassum fulvellum
• ISSN: 2288-1174; 2383-9651

Purpose : Melanin is a brown or black pigment that determines the colour of the skin and hair, which can protect the skin from UV. By the way, melanin can cause various disorders such as melasma, freckles, and even melanoma when it is overproduced. Therefore, many studies have been performed on natural products to improve these symptoms. In this study, four types of brown seaweeds, Saccharina japonica, Hizikia fusiforme, Undaria pinnatifida, and Sargassum fulvellum, were fermented using Lactobacillus sakei, and analysed for their whitening effects on B16F10 melanocytes. Methods : The cytotoxicity of fermented seaweeds extracts on RAW 264.7 cells and B16F10 cells was evaluated by the WST-1 assay. The Griess reaction was performed to measured the produced nitric oxide (NO) concentration. To identify the whitening activity of four kinds of seaweeds fermentation extracts, in vitro tyrosimase activity and melanin content were analyzed. Results : High concentrations of S. japonica and S. fulvellum induced mild cytotoxicity in RAW 264.7 cells, but the other samples showed no significant toxicity in both RAW 264.7 and B16F10 cells. The four fermented seaweed extracts significantly inhibited the production of LPS-induced NO in RAW 264.7 cells, with H. fusiforme and U. pinnatifida showing stronger anti-inflammatory activity than that of others. The inhibitory effect of in vitro tyrosinase activity was in the order of U. pinnatifida, S. fulvellum, H. fusiforme, S. japonica. The melanin synthesis induced by -MSH was also inhibited by the four kinds of agents, which was in consistent with the tyrosinase assay. Conclusion : These results are thought to be due to the increased concentration of various active substances during the fermentation process, and it is necessary to analyse which substances were increased in this study in the future. Based on these results, four kinds of fermented seaweeds extracts have the potential to be used as whitening functional cosmetic ingredients.