Thioacetamide로 유발된 간손상 동물모델에 인진호 추출물의 간보호 효과

• Published in: Yaghag-hoi-ji Vol. 65; no. 3; pp. 201 - 208
• Main Authors: 김민주(Min Ju Kim), 이진아(Jin A Lee), 이지혜(Ji Hye Lee), 신미래(Mi-Rae Shin), 노성수(Seong-Soo Roh)
• Format: Journal Article
• Language: Korean
• Published: The Pharmaceutical Society Of Korea 30.06.2021; 대한약학회
• Subjects: 약학; thioacetamide; Artemisia Capillaris Herba; liver injury
• ISSN: 0377-9556; 2383-9457
• DOI: 10.17480/psk.2021.65.3.201

This study investigated the hepatoprotective effect of Artemisia Capillaris Herba water extract (AC) in athioacetamide (TAA) induced acute liver damage model using Sprague-Dawley (SD) rats. The in vitro antioxidant activityof AC was measured by quantitating total polyphenol content, total flavonoid content, and DPPH and ABTS free radicalscavenging. Our animal experiment was conducted as follows: AC (100 and 200 mg/kg body weight, PO) and silymarin(Sily 100; 100 mg/kg body weight, PO) were administered for 3 days, concurrent with induction of liver injury (TAA,200 mg/kg body weight, IP). Serum and liver tissue were collected after 3 days of drug administration. AC treatmentsignificantly reduced serum glutamic oxaloacetic transaminase (GOT), glutamate pyruvate transaminase (GPT),myeloperoxidase (MPO), and ammonia levels. It also effectively decreased protein level expression of NF-ĸBp65, p-IĸBα,COX-2, iNOS, TNF-α, IL-6, and IL-1β in liver tissue, while increasing ZO-1, occludin, claudin-1, claudin-3, and claudin-4 expression. These results suggest that AC treatment can attenuate inhibition of the NF-ĸB pathway, and enhancement ofthe tight junction (TJ) pathway in TAA-induced liver injury. Therefore, AC displays potential for use as a naturaltherapeutic drug. KCI Citation Count: 0