이형접합 Pinb-D1 유전자형이 밀가루 이화학적 특성 및 가공 적성에 미치는 영향

• Published in: Korean journal of breeding Vol. 52; no. 2; pp. 131 - 139
• Main Author: 최창현(Chang-Hyun Choi), 박진희(Jin Hee Park), 모영준(Youngjun Mo), 김경민(Kyung-Min Kim), 김경훈(Kyung-Hoon Kim), 강천식(Chon-Sik Kang), 박태일(Tae-Il Park), 조성우(Seong-Woo Cho), 박철수(Chul Soo Park)
• Format: Journal Article
• Language: Korean
• Published: 한국육종학회 30.06.2020; The Korean Breeding Society
• Subjects: 농학; Korean wheat; end-use quality; physicochemical properties; puroindolines
• ISSN: 0250-3360; 2287-5174
• DOI: 10.9787/KJBS.2020.52.2.131

An advanced F8 population was derived from a cross between the hard wheat cultivar “Keumkang” carrying Pinb-D1b and thesoft wheat cultivar “Olgeuru” carrying Pinb-D1a. A breeding line named “Hetero”, which exhibited the heterozygous-like Pinb-D1a/Pinb-D1bgenotype, was selected by sequence analysis and KASP (Kompetitive Allele Specific PCR) assay. Physicochemical and processing characteristicsof flour were tested in the Hetero line and compared with the two parental cultivars for two years. Hetero display a Glu-1 composition similarto Keumkang and a Glu-3 composition intermediate between Keumkang and Olgeuru. Contrary to the expectation that Hetero carrying thePinb-D1a/Pinb-D1b genotype would exhibit an intermediate phenotype between the two parents, its overall flour physicochemical characteristicswere more similar to Keumkang than to Olgeuru. The flour yield of Hetero (71.6%) was lower than that of Keumkang (74.5%). However,the flour particle size (73.3 μm) and damaged starch content (4.6%) of Hetero were similar to those of Keumkang (71.9 μm and 4.5%). The protein content (16.5%) and sodium dodecyl sulfate-sedimentation volume (72.8 mL) of Hetero were higher than those of Keumkang(14.0% and 57.5 mL) and Olgeuru (11.4% and 45.5 mL). The mixograph water absorption (68.7%) and mixing tolerance (17.7 mm) valuesof Hetero were higher than those of Keumkang (65.6% and 15.9 mm) with the same mixing time of 3.9 min. The bread loaf volume ofHetero was lower than that of Keumkang (866.7 vs. 894.7 mL). The cooked noodles hardness of Hetero was higher than that of Keumkang(5.9 vs. 4.7 N). KCI Citation Count: 3