염생식물 도깨비고비의 항산화 및 항염증 효과

• Published in: Ocean and polar research Vol. 43; no. 3; pp. 113 - 126
• Main Authors: 김현모, 김호준, 공창숙, 이봉호, 심현보, 서영완, Kim, Hyunmo, Kim, Hojun, Kong, Chang-Suk, Lee, Bong Ho, Sim, Hyun-Bo, Seo, Youngwan
• Format: Journal Article
• Language: Korean
• Published: 한국해양과학기술원 01.09.2021
• Subjects: 해양학; anti-inflammatory; Cyrtomium falcatum; antioxidant; halophyte; reactive oxygen species (ROS)
• ISSN: 1598-141X; 2234-7313
• DOI: 10.4217/OPR.2021.43.3.113

In the present study, the halophyte C. falcatum extract and its solvent fractions (n-hexane, 85% aqueous methanol, n-butanol, and water) were evaluated for antioxidant and anti-inflammatory activities. Antioxidative ability was measured by DPPH radical, intracellular reactive oxygen species (ROS) and peroxynitrite scavenging, DNA oxidation inhibition, and ferric reducing antioxidant power (FRAP). For DPPH radical and peroxynitrite scavenging, DNA oxidation inhibition, and FRAP, 85% aq.MeOH and n-BuOH fractions showed significant scavenging activity. For production of intracellular ROS in HT-1080 cells, 85% aq.MeOH fraction showed the highest scavenging activity. In addition, anti-inflammatory activity was also assessed by measuring the inhibitory effect against mRNA expression of pro-inflammatory factors (NO, IL-1β, IL-6 and COX-2) in LPS-stimulated Raw 264.7 macrophages. For NO production, crude extract exhibited a strong inhibitory effect at a concentration of 100 ㎍/ml. For mRNA expression of pro-inflammatory cytokines (IL-1β, IL-6, and COX-2), n-BuOH greatly suppressed expression levels of IL-1β and IL-6 at 100 ㎍/ml concentration while 85% aq. MeOH fraction significantly inhibited that of COX-2 even at 100 ㎍/ml. These results suggest that C. falcatum may be used as a potential source for the development of a natural antioxidant or anti-inflammatory agent.