식이유도 비만 마우스에서 대마 추출물의 체중 감량 효과에 대한 예비 연구

• Published in: Singyŏng chŏngsin ŭihak Vol. 59; no. 3; pp. 260 - 267
• Main Author: 장유진(Yoo Jin Jang), 임신원(Shinn-Won Lim), 우숙영(Sook-Young Woo), 김수연(Su Yeon Kim), 김도관(Doh Kwan Kim)
• Format: Journal Article
• Language: Korean
• Published: 대한신경정신의학회 31.08.2020
• Subjects: 정신과학; Obesity; Diet-induced obese mouse; Glucose metabolism homeostasis; Cannabis; Weight loss; 체중감소; 대마; 식이유도 비만 마우스; 포도당대사 항상성; 비만
• ISSN: 1015-4817; 2289-0963
• DOI: 10.4306/jknpa.2020.59.3.260

Objectives This study aimed to investigate the pharmacological effect of cannabis extract on weight loss in diet-induced obese mice. Methods A total of 12 C57BL/6 male mice (Orient Bio), aged 6 weeks, were fed a high-fat diet for 13 weeks to construct a diet-induced obesity model. During the following 5 weeks, diet-induced obese mice were daily administered cannabis extract or sesame seed oil orally along with the high-fat diet. The body weight of each subject was measured weekly. Venous blood was drawn for biochemistry, enzyme-linked immunoassay, and oral glucose tolerance test before and after treatment. Body fat was measured by dual-energy X-ray absorptiometry, and the mesenteric adipose tissue was also measured after sacrifice. We used exact Wilcoxon’s two-sample analyses and generalized estimating equations to test the differences between the cannabis-treated group and control. Results There was significant weight loss (p=0.009) observed in the cannabis-treated mice compared to the control group after 5 weeks of treatment. High-fat diet-induced glucose intolerance in the cannabis-treated group was significantly ameliorated (p=0.032), whereas there were no profound differences between the two groups in terms of other physiological markers, including corticosterone level. Conclusion This study shows that orally administered cannabis extract had a pharmacological effect of weight loss in diet-induced obese mice. This weight loss might be attributed to an increase in energy expenditure and regulation of glucose homeostasis. KCI Citation Count: 1