Use of Antibody Displayed Phage for the Detection of Dextran Using a Dipstick Assay and Transmission Electron Micrograph

• Published in: Journal of microbiology and biotechnology Vol. 16; no. 8; pp. 1316 - 1319
• Main Authors: Kim Du-Woon, Day Donal F
• Format: Journal Article
• Language: Korean
• Published: 한국미생물·생명공학회 01.08.2006
• Subjects: 생물학; Dextran; phage display; electron microscopy
• ISSN: 1017-7825; 1738-8872

An antibody displayed phage collection (SBAE-2R), screened from a human synthetic phage antibody library (Fab 21ox), was used for the determination of dextran. The dextran-binding affinity was determined by serologically specific transmission electron microscopy (TEM) and a paper dipstick assay. The phage collection was distributed over the dextrancoated grids with 39$\pm$25 phages/$\mu$m$^2$ on the grids. Phages were not seen on dextran-coated grids exposed to the Fab 2lox phage library. The phage collection (SBAE-2R) produced 54$\pm$3 color normalized intensity (N.I.) from 125 ppm to 1,000 ppm of dextran and 5$\pm$1 (N.I.) for 63 ppm of dextran in a paper dipstick assay. This research extends the analytical options for dextran analysis by antibody displayed phage with a minimum of equipment usage.