결빙억제제의 종류, 농도 및 침지시간이 참전복 Haliotis discus hannai 발생배의 생존활성에 미치는 영향

• Published in: Balsaeng'gwa saengsig Vol. 15; no. 4; pp. 301 - 308
• Main Authors: 정종균, 임한규, 손맹현, 김종현, 정민환, 장영진, Chung, Jong-Kyun, Lim, Han-Kyu, Son, Maeng-Hyun, Kim, Jong-Hyun, Jeong, Min-Hwan, Chang, Young-Jin
• Format: Journal Article
• Language: Korean
• Published: 한국발생생물학회 01.12.2011
• Subjects: 생물학; Embryo; Immersion test; Abalone; Haliotis discus hannai; CPA; Cryopreservation; Development
• ISSN: 2465-9525; 2465-9541

The tolerance evaluation for abalone Haliotis discus hannai embryos was performed using different concentrations of cryoprotective agents (CPAs): dimethyl sulfoxide, ethylene glycol and propylene glycol added to 0.2 M sucrose, respectively. 4-cell, trochophore and veliger were exposed in each CPA with different concentration for 10, 20 and 30 minutes of immersion time. Developmental rates were increased with decreased concentration of every CPA and decreased immersion time, and differed from types of CPA. Developmental rates of veliger in all the CPAs were higher than those of 4-cell and trochophore. The developmental rates and larval activity indices in ethylene glycol were comparatively higher than those in other CPAs and the effective CPA and its concentration for the cryopreservation of the abalone embryos was suggested as 2.0 M ethylene glycol with equilibration time of 30 minutes. 결빙억제제(CPA)의 종류, 농도 및 침지시간이 참전복 Haliotis discus hannai 발생배의 생존활성에 미치는 영향을 조사하였다. 4세포, 담륜자 및 피면자를 dimethyl sulfoxide, ethylene glycol, propylene glycol 농도별로 10분, 20분 및 30분간 침지하였다. CPA 침지 후 각 발생배의 발생진행률은 각 CPA 농도가 낮을수록, CPA 침지시간이 짧을수록 높았다. 또한, 피면자에서 비틀림전까지의 발생진행률은 4세포에서 담륜자까지의 것보다 높았다. CPA 침지 후 발생진행률 및 유생활성지수는 ethylene glycol에서 가장 높았다. 참전복 발생배의 냉동보존 효과를 높일 수 있는 CPA 및 농도는 ethylene glycol 2.0 M, 평형시간은 30분인 것으로 추정된다.