miR-4708의 multidrug resistance protein-1 발현 조절을 통한 paclitaxel 민감성 증가

• Published in: 대한구강악안면병리학회지, 41(2) pp. 55 - 62
• Main Authors: 최장열, 유지원, 안상건
• Format: Journal Article
• Language: Korean
• Published: 대한구강악안면병리학회 30.04.2017
• Subjects: 치의학
• ISSN: 1225-1577

Multidrug resistance (MDR) remains one of the most significant obstacles in various cancer treatment, and this process often involves dysregulation of the number of micro-RNAs. The aim of this study was to explore the role of miR-4708 on the regulation of MDR-1 expression and the regulation of multidrug resistance (MDR) to chemotherapeutic drugs. Luciferase reporter assays demonstrated tha t miR-4708 directly binds MDR-1 3’-UTR and down-regulated reporter luciferase activity. The mRNA and protein expression levels of MDR1 were significantly decreased following miR-4708 overexpression. Additionally, the accumulation of rhodamine-123 in paclitacel resistant FaDu cells following miR-4708 transfection was significantly increased compared with control, indicating that the efflux capacity was reduced. These results demonstrated that miR-4708 could be involved in the regulation of MDR via targeting MDR-1 and may provide a potential strategy for reversing drug resistance in oral cancer. KCI Citation Count: 0