소 난소 저온 보존이 난자의 체외 발달에 미치는 영향

• Published in: Journal of animal reproduction & biotechnology (Online) pp. 185 - 190
• Main Authors: 김성우, 김민수, 김찬란, 김동교, 김남태, 성환후(농촌진흥청 국립축산과학원
• Format: Journal Article
• Language: Korean
• Published: 사단법인 한국동물생명공학회 30.09.2016
• Subjects: 축산학
• ISSN: 2671-4639; 2671-4663

During the ovary preservation in low temperature, the cumulus oocyte complexes(COCs) lose their developmental competences after in vitro fertilization. We used phosphate-buffered saline (PBS) as a basic solutions of at various temperatures (25, 15 or 5 ℃) and supplemented them with 1mM glucose and 0.5mM glutamine as a source of carbohydrate metabolites. After recovery of COCs and in vitro fertilization, a significantly higher number of oocytes developed into blastocysts. The developmental competence of embryos that were originated from ovaries preserved at 15 ℃ was increased compared to those of 25 or 5 ℃. The maturation rate of oocytes was not differed between 24 and 36 h at 15 ℃ but showed lower than control group (71% versus 78%). In vitro-fertilized oocytes from ovaries stored at 25 ℃ for 24 h or at 5 ℃ for 24 h had a significantly decreased developmental potentials, but at 15 ℃ did not (27% versus 29% of blastocysts to develop into day 8). With these results, bovine ovaries can be preserved at 15 ℃ for 36 h without decreasing developmental capacity of in vitro-fertilized oocyte at least to the blastocyst stage. This information provides valuable information of preserving ovaries for embryo transfer or in vitro embryo production. KCI Citation Count: 0