A Simple Method for Cat Bone Marrow-derived Mesenchymal Stem Cell Harvesting

Bone marrow (BM) cell harvesting is a crucial element in the isolation of mesenchymal stem cells (MSCs). A simple method for harvesting cat BM cells is described. The results show that a large number of BM cells can rapidly be harvested from the cat by this simple procedure. MSCs prepared by density...

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Bibliographic Details
Published inJournal of animal reproduction & biotechnology (Online) pp. 127 - 131
Main Authors Jin Guang Zhen, Lee Young Soo, Choi Eugene, 조규완, 공일근
Format Journal Article
LanguageEnglish
Published 사단법인 한국동물생명공학회 30.06.2008
Subjects
축산학
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ISSN2671-4639
2671-4663

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Summary:Bone marrow (BM) cell harvesting is a crucial element in the isolation of mesenchymal stem cells (MSCs). A simple method for harvesting cat BM cells is described. The results show that a large number of BM cells can rapidly be harvested from the cat by this simple procedure. MSCs prepared by density-gradient method were spindle-shaped morphology with bipolar or polygonal cell bodies and strongly positive for CD9 and CD44 and negative for CD18 and CD45-like. They were capable of differentiation to adipocytic and osteocytic phenotypes when exposed to appropriate induction media. The advantages of this method are its rapidity, simplicity, low invasiveness, and low donor attrition and good outcome. KCI Citation Count: 0
Bibliography:G704-001405.2008.23.2.001
ISSN:2671-4639
2671-4663