Regulation of [ 3 H]Norepinephrine Release by Opioids in Human Cerebral Cortex

• Published in: The Korean journal of physiology & pharmacology Vol. 7; no. 1; pp. 1 - 3
• Main Authors: Woo, Ran-Sook, Shin, Byoung-Soo, Kim, Chul-Jin, Shin, Min-Soo, Jeong, Min-Suk, Zhao, Rong-Jie, Kim, Kee-Won
• Format: Journal Article
• Language: Korean
• Published: 대한생리학회-대한약리학회 01.01.2003; The Korean Journal of Physiology & Pharmacology Editorial Office
• Subjects: Opioid receptors; Norepinephrine release; Human cerebral cortex
• ISSN: 1226-4512; 2093-3827

To investigate the receptors mediating the regulation of norepinephrine (NE) release in human cerebral cortex slices, we examined the effects of opioid agonists for ${\mu}$-, ${\delta}$-, and ${\kappa}$-receptors on the high potassium (15 mM)-evoked release of [$^3H$]NE. [$^3H$]NE release induced by high potassium was calcium-dependent and tetrodotoxin-sensitive. [$D-Pen^2$, $D-Pen^5$]enkephalin (DPDPE) and deltorphin II (Delt II) inhibited the stimulated release of norepinephrine in a dose-dependent manner. However, Tyr-D-Ala-Gly-(Me)Phe-Gly-ol and U69,593 did not influence the NE release. Inhibitory effect of DPDPE and Delt-II was antagonized by naloxone, naltrindole, 7-benzylidenaltrexone and naltriben. These results suggest that both ${\delta}_1$ and ${\delta}_2$ receptors are involved in regulation of NE release in human cerebral cortex.