미국삼 ( Panax quinquefolium ) 에서 분리한 뿌리썩음병균 Cylindrocarpon destructans의 후막포자 생성 및 분리 ( Production and Isolation of Chlamydospores in Cylindrocarpon destuctans Causing Root Rot of panax quinquefolium )

• Published in: Journal of ginseng research Vol. 22; no. 4; pp. 304 - 309
• Main Authors: 조대휘, 유연현, 오승환, Jennifer L. Parke
• Format: Journal Article
• Language: Korean
• Published: 고려인삼학회 1998
• Subjects: Cylindrocarpon destructans; Panax quinquefolium; chlamydospore; macroconidia
• ISSN: 1226-8453; 2093-4947

Incubation condition affecting the chlamydospore formation and isolation from mycelia and conidia of Cylindrocarpon destructanse (isolate ACY-9701), isolated from the root rot lesion of the American ginseng (Panax quinquefolium) was investigated. Chlamydospores were formed from mycilia but not from conidia on the Czapek-Dox agar without carbon or nitrogen source after 20 days incubation at 2$0^{\circ}C$. In the medium added with nitrogen and carbon sources, immatured chlamy-dospore-like cells were formed from microconidia and mycelia as well. Immatured chlamydospore-like cells were formed from mycelia as well as microconidia In corn, kidney bean, and pea root extracts after 20 days incubation at 20"C, while typical chlamydospores were formed from both of them in the root extract of Panax quinquefolium. The 3.6 log chlamydospore/mm" was converted from microconidia in the medium, which was equal to 2.5% conidia formed. Under the light condition (251.1 pmol/m" sec, 12 hrs dark and light cycle), 4.2 log/mm" of chlamydospores were converted from interracially or terminal cells of macroconidia, which was 4.0% of macroconidia produced on Potato dextrose agar (PDA). When mycelia and microconidia were stored at -7$0^{\circ}C$ for 32 days and incubated on PDA after thawing at room temperature to isolate chlamydospores from them, microconidia and mycelia were still alive. Meanwhile, microconidial lysis was found after heating them at 32$^{\circ}C$ for 7 days, but the chlamydospores converted from macroconidia were not lysed up to 13 days at 32"C. to 13 days at 32"C.ot;C.