고로쇠 Coumarinolignan의 β-Cyclodextrin 포접화합물 제조 및 암세포증식 억제활성

• Published in: 생약학회지 Vol. 46; no. 2; pp. 133 - 139
• Main Authors: 김현정(Huyn Jung Kim), 임순호(Soon Ho Yim), 정다운(Da Woon Jung), 윌리엄스다렌(Darren R. Williams), 게클러커트(Kurt E. Geckeler), 김경근(Kyung Keun Kim), 신부안(Boo Ahn Shin), 이익수(Ik Soo Lee)
• Format: Journal Article
• Language: Korean
• Published: 한국생약학회 2015
• Subjects: β-Cyclodextrin inclusion complex; Anti-proliferation; Acer mono; Coumarionolignans; Cleomiscosins; {\beta}$-Cyclodextrin inclusion complex
• ISSN: 0253-3073; 2288-9299

Two coumarinolignans, cleomiscosins C (1) and D (2) were isolated from the heartwood of Acer mono, together with four compounds, 5-O-methyl-(E)-resveratrol-3-O-${\beta}$-D-glucopyranoside (3), 5-O-methyl-(E)-resveratrol-3-O-${\beta}$-D-apiofuranosyl-(1$\rightarrow$6)-${\beta}$-D-glucopyranoside (4), scopoletin (5), and (E)-resveratrol-3-O-${\beta}$-D-glucopyranoside (6). Of them, cleomiscosins C (1) and D (2) were applied to preparing inclusion complex molecules with ${\beta}$-cyclodextrin (${\beta}$-CD) to improve the very poor solubility in cell media. The CD complexes of 1 and 2 exhibited an enhancement of water solubility which is feasible to measure their cytotoxicity using a spectrophotometer in a cell-based assay. Anti-proliferative activity of these complex molecules was successfully estimated on HCT116 human colon cancer cells, and cleomiscosin D (2) showed anti-proliferative effects at the concentration of 1.95~31.2 ${{\mu}g}$/mL in a dose-dependent manner.