물벼룩 지수식 배양방법과 물벼룩 연속식 배양장치를 이용한 연속식 배양방법의 비교

• Published in: 환경분석과 독성보건 Vol. 28; no. 1; pp. 50 - 60
• Main Authors: 최동진, Dong-jin Choi
• Format: Journal Article
• Language: Korean
• Published: 한국환경분석학회 31.03.2025
• Subjects: Acute toxicity; continuous culture apparatus; Daphnia magna; EC 50; ecotoxicity; 화학
• ISSN: 2672-0175; 2672-1139
• DOI: 10.36278/jeaht.28.1.50

The ecotoxicity value of unknown harmful substances contained in effluent was calculated by determining the health status of Daphnia magna (water fleas) through a standard reference toxicity test (ES 04705.1b). For this test, the Daphnia magna was cultured with static non-renewal culture and the Daphnia magna neonate was separated, which was time-consuming. This problem was solved by developing a continuous water flea culture apparatus and a continuous culture method. Standard reference toxicity tests were performed to confirm the health status of static non-renewal cultured Daphnia magna and continuous cultured Daphnia magna. We found that the average test value (EC 50 ) for neonate separated from static non-renewal cultured Daphnia magnawas 1.020 mg/L. Additionally, the average test value (EC 50 ) for neonate separated in natural flow from continuous cultured Daphnia magna was 0.992 mg/L. It was confirmed that the EC 50 values for potassium dichromate were in the satisfactory range of 0.9 to 2.1 mg/L, so it was valid for ES 04705.1b and ISO 6341:2012. In conclusion, there is a high chance for saving time and effort for toxicity evaluation when the continuous culture apparatus is used.