마치현(Portulaca oleracea L.) 추출물의 항염 및 항소양 작용: LPS 처리로 염증을 유도한 Raw264.7세포와 각질형성세포, NC/Nga 마우스, Hairless 마우스에 대한 결과

• Published in: Chʻŏnsik mit alrerugi Vol. 31; no. 3; pp. 199 - 206
• Main Authors: 임윤영, Yun Young Lim, 김형미, Hyeong Mi Kim, 박원석, Won Seok Park, 김정환, Jeong Hwan Kim, 신홍주, Hong Ju Shin, 김명남, Myeong Nam Kim, 김범준, Beom Joon Kim
• Format: Journal Article
• Language: Korean
• Published: 대한천식알레르기학회 30.09.2011
• Subjects: Atopic dermatitis; Portulaca oleracea L; Pruritus; 임상병리학
• ISSN: 1226-8739

Background: Portulaca oleracea L. is known to have detoxification and antibacterial effects. Inflammatory responses in LPS-treated Raw264.7 cells include the secretions of NO, PGE2 and numerous cytokines. Atopic dermatitis is a chronic inflammatory skin disease. This study was performed to identify the antiinflammatory or anti-pruritic effects of Portulaca oleracea L. extract in LPS-treated Raw264.7 cells and keratinocytes and the skin of NC/Nga mice with atopic dermatitis, as well as hairless mice with pruritus. Methods: After the treatment of different concentrations for Portulaca oleracea L. extract in LPS-treated Raw264.7 cells and keratinocytes, the levels of NO, PGE2 and proinflammatory cytokines were measured in the media. Skin tissues of all Nc/Nga mice were subjected to H&E staining and toluidine blue staining. An antipruritic effect was defined as the number of scratching behaviors of the hairless SKH-1 mice treated with compound 48/80. Results: LPS- and IFN-γ-treated Raw264.7 cells decreased NO and PGE2 productions compared to non-treated Raw264.7 cells. H&E or toluidine blue staining revealed that the thicknesses of the epidermis and dermis were reduced in NC/Nga mice with atopic dermatitis treated with Portulaca oleracea L. extract. However, Itching behaviors in hairless mice treated with Portulaca oleracea L. extract were not decreased compared to non-treated hairless mice. Conclusion: The results of this study suggest that Portulaca oleracea L. extract could be a new therapeutic agent for atopic dermatitis. (Korean J Asthma Allergy Clin Immunol 2011;31:199-206)