Curcumin ameliorates TNF-α-induced ICAM-1 expression and subsequent THP-1 adhesiveness via the induction of heme oxygenase-1 in the HaCaT cells

• Published in: BMB reports Vol. 46; no. 8; pp. 410 - 415
• Main Authors: Youn, Gi Soo, Kwon, Dong-Joo, Ju, Sung Mi, Choi, Soo Young, Park, Jinseu
• Format: Journal Article
• Language: Korean
• Published: 생화학분자생물학회 30.08.2013
• Subjects: Curcumin; HO-1; ICAM-1; Inflammation; Keratinocyte; Keratinocyte; Curcumin; HO-1; ICAM-1; Inflammation
• ISSN: 1976-6696; 1976-670X

Adhesion molecules such as ICAM-1 are important in the infiltration of leukocytes into the site of inflammation. In this study, we investigated the inhibitory effects of curcumin on ICAM-1 expression and monocyte adhesiveness as well as its underlying action mechanism in the TNF-α-stimulated keratinocytes. Curcumin induced expression of heme oxygenase-1 (HO-1) in the human keratinocyte cell line HaCaT. In addition, curcumin induced Nrf2 activation in dose- and time-dependent manners in the HaCaT cells. Curcumin suppressed TNF-α- induced ICAM-1 expression and subsequent monocyte adhesion, which were reversed by the addition of tin protoporphyrin IX (SnPP), a specific inhibitor of HO-1, or HO-1 knockdown using siRNA. Furthermore, Nrf2 knockdown using siRNA reversed the inhibitory effect of curcumin on the TNF-α-induced ICAM-1 expression and adhesion of monocytes to keratinocytes. These results suggest that curcumin may exert its anti-inflammatory activity by suppressing the TNF-α-induced ICAM-1 expression and subsequent monocyte adhesion via expression of HO-1 in the keratinocytes. [BMB Reports 2013; 46(8): 410-415]