자양대보탕(滋養大補湯)의 발효 전·후 항염증, 항암, 항산화 효과 비교 연구

• Published in: 大韓本草學會誌 Vol. 38; no. 5; pp. 49 - 60
• Main Authors: 김주영, Juyoung Kim, 홍은서, Eunseo Hong, 박용기, Hyo Won Jung, 정효원, Yong-ki Park
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 30.09.2023
• Subjects: anti-inflammation; anticancer; antioxidation; fermentation; Jayangdaebo-tang; fermentation; anti-inflammation; antioxidation; Jayangdaebo-tang; anticancer
• ISSN: 1229-1765

Objectives : In this study, it was investigated the anti-inflammatory, anticancer, and antioxidant effects of Jayangdaebo-tang (JDT) consisting of twelve herbs before and after fermentation. Methods : JDT extract was fermented using the Lactoplantibacillus plantanum (JDT-L), Bacillus subtilis (JDT-B), and L. plantanum plus B. subtilis (JDT-L+B). The effects of each extract were measured in LPS-stimulated RAW264.7 cells, MCF-7 breast cancer and A549 lung cancer cells, and H 2 O 2 -stimulated HepG2 cells. Results : The extracts of JDT-L, JDT-B and JDT-L+B at 1 ㎎/㎖ decreased significantly the levels of nitric oxide (NO) in LPS-treated RAW264.7 cells and also inhibited the expression of iNOS and COX-2, and the phosphorylation of ERK and NF-κB. The JDT-L+B extract decreased significantly the expression of apoptotic proteins, Bax, cleaved caspase-3, and PARP in MCF-7 and A549 cancer cells. The JDT-L, JDT-B and JDT-L+B extracts increased significantly the cell viability in H 2 O 2 -stimulated HepG2 cells and the JDT-L+B extract decreased significantly the expression of SOD, catalase, HO-1, and NRF-2. Among fermented JDT extracts, JDT-L+B was the best effective on response of macrophage inflammation, cancer cell apoptosis, and liver cell damage. Conclusions : Our results were suggested that the fermentation can be used as a useful way to enhance the biological activity of JDT.