Identification of SUMOylated proteins in neuroblastoma cells after treatment with hydrogen peroxide or ascorbate

The small ubiquitin-like modifier (SUMO) proteins have been implicated in the pathology of a number of diseases, including neurodegenerative diseases. The conjugation machinery for SUMOylation consists of a number of proteins which are redox sensitive. Here, under oxidative stress (100 μM hydrogen p...

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Bibliographic Details
Published inBMB reports Vol. 43; no. 11; pp. 720 - 725
Main Author Grant, Melissa M
Format Journal Article
LanguageKorean
Published 생화학분자생물학회 30.11.2010
Subjects
Oxidative stress
Proteomics
SIM
SUMOylation
Oxidative stress
SUMOylation
SIM
Proteomics
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Summary:The small ubiquitin-like modifier (SUMO) proteins have been implicated in the pathology of a number of diseases, including neurodegenerative diseases. The conjugation machinery for SUMOylation consists of a number of proteins which are redox sensitive. Here, under oxidative stress (100 μM hydrogen peroxide), antioxidant (100 μM ascorbate) or control conditions 169 proteins were identified by electospray ionisation fourier transform ion cyclotron resonance mass spectrometry. The majority of these proteins (70%) were found to contain SUMOylation consensus sequences. From the remaining proteins a small number (12%) were found to contain possible SUMO interacting motifs. The proteins identified included DNA and RNA binding proteins, structural proteins and proteasomal proteins. Several of the proteins identified under oxidative stress conditions had previously been identified as SUMOylated proteins, thus validating the method presented. [BMB reports 2010; 43(11): 720-725]
Bibliography:Korean Society for Biochemistry and Molecular Biology
KISTI1.1003/JNL.JAKO201001039069147
ISSN:1976-6696
1976-670X