A Dual Selection Marker Transformation System Using Agrobacterium tumefaciens for the Industrial Aspergillus oryzae 3.042

Currently, the genetic modification of Aspergillus oryzae is mainly dependent on protoplast-mediated transformation (PMT). In this study, we established a dual selection marker system in an industrial A. oryzae 3.042 strain by using Agrobacterium tumefaciens-mediated transformation (ATMT). We first...

Full description

Saved in:
Bibliographic Details
Published inJournal of microbiology and biotechnology Vol. 29; no. 2; pp. 230 - 234
Main Authors Sun, Yunlong, Niu, Yali, He, Bin, Ma, Long, Li, Ganghua, Tran, Van-Tuan, Zeng, Bin, Hu, Zhihong
Format Journal Article
LanguageKorean
Published 한국미생물생명공학회 28.02.2019
Subjects
Agrobacterium tumefaciens-mediated transformation
Aspergillus oryzae
pyrithiamine
uridine/uracil auxotrophy
pyrithiamine
Agrobacterium tumefaciens-mediated transformation
Aspergillus oryzae
uridine/uracil auxotrophy
Online AccessGet full text

Cover

More Information
Summary:Currently, the genetic modification of Aspergillus oryzae is mainly dependent on protoplast-mediated transformation (PMT). In this study, we established a dual selection marker system in an industrial A. oryzae 3.042 strain by using Agrobacterium tumefaciens-mediated transformation (ATMT). We first constructed a uridine/uracil auxotrophic A. oryzae 3.042 strain and a pyrithiamine (PT)-resistance binary vector. Then, we established the ATMT system by using uridine/uracil auxotrophy and PT-resistance genes as selection markers. Finally, a dual selection marker ATMT system was developed. This study demonstrates a useful dual selection marker transformation system for genetic manipulations of A. oryzae 3.042.
Bibliography:The Korean Society for Applied Microbiology
KISTI1.1003/JNL.JAKO201912937050566
ISSN:1017-7825
1738-8872