Development of DNA Chip Microarrayer

• Published in: Journal of microbiology and biotechnology Vol. 10; no. 1; pp. 21 - 26
• Main Authors: Yoon, Sung-Ho, Choi, Jong-Gil, Lee, Sang-Yup
• Format: Journal Article
• Language: Korean
• Published: 한국미생물생명공학회 01.02.2000
• Subjects: microarrayer; DNA chip; high density microarray
• ISSN: 1017-7825; 1738-8872

A microarrayer system was developed mainly for manufacturing DNA chips. The 3-axis robot was designed to automatically collect samples from 96-or 384-well microtiter plates using up to 16 simultaneously moving pens and to deposit them on a surface-modified slide glass. This is followed by a wash/dry operation in a clean station. The cycle is repeated with a new set of samples, This system can deposit cDNA or oligonucleotides with spot intervals of $150{\;}\mu\textrm{m}$ and the spot size of $80\mu\textrm{m}$, thus allowing a high density DNA chip containing about 5,000 spots per $\textrm{cm}^2$. The entire procedure is controlled by the Visual C++ program that was written in our laboratory by using a personal computer with Pentium 100 CPU.