Large-Scale Fermentation for the Production of Teicoplanin From a Mutant of Actinoplanes teichomyceticus

• Published in: Journal of microbiology and biotechnology Vol. 15; no. 4; pp. 787 - 791
• Main Authors: Jae Chan Lee, Jung Won Min, Dong Jin Park, Kwang Hee Son, Ki Hong Yoon, Hae Ryong Park, Young Soo Park, Mu Gil Kwon, Jung Min Lee, Chang Jin Kim
• Format: Journal Article
• Language: Korean
• Published: 한국미생물생명공학회 30.08.2005
• Subjects: Actinoplanes teichomyceticus; fermentation; mutagenesis; mutation; teicoplanin; teicoplanin; mutation; Actinoplanes teichomyceticus; fermentation; mutagenesis
• ISSN: 1017-7825; 1738-8872

Mutation and its pilot-scale fermentation were conducted for the production of teicoplanin from Actinoplanes teichomyceticus. The fermentation medium was optimized by replacement and Plackett-Burman experimental design. A maximum production of 1,500 mg/l teicoplanin was obtained by pilot-scale fermentation in an optimized medium containing (g/l): 30 g maltodextrin, 5 g glucose, 5 g yeast extract, 5 g soybean meal, 0.5 g $MgSO_4{\cdot}7H_2O$, 0.1 g NaCl, 0.1 g $CaCl_2{\cdot}2H_2O$, and 50 g Diaion HP-20. The production of teicoplanin was improved 3-fold from the parental strain by mutation, media optimization, and fermentation, and laboratory-scale fermentation was successfully demonstrated in a pilot-scale fermenter for the industrial production of teicoplanin.