Evaluation of the inhibitory effect of Gynostemma pentaphyllum extracts on CYP450 enzyme activities using LC-MS/MS

Gynostemma pentaphyllum (Thunb.) Makino extract, a natural product with a history of traditional use, has gained attention for its potential health benefits. This study aimed to investigate its effects on key cytochrome P450 (CYP) enzymes using LC-MS/MS. Human liver microsomes and cDNA-expressed CYP...

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Bibliographic Details
Published inMass spectrometry letters Vol. 14; no. 3; pp. 116 - 119
Main Authors Jun Sang Yu, Young Seok Ji, So Young Jo, Xiang-Lan Piao, Hye Hyun Yoo
Format Journal Article
LanguageKorean
Published 30.09.2023
Subjects
LC-MS/MS
Gynostemma pentaphyllum
cytochrome P450
herb-drug interaction
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Summary:Gynostemma pentaphyllum (Thunb.) Makino extract, a natural product with a history of traditional use, has gained attention for its potential health benefits. This study aimed to investigate its effects on key cytochrome P450 (CYP) enzymes using LC-MS/MS. Human liver microsomes and cDNA-expressed CYP2C8, CYP2C9, CYP2C19, and CYP3A4 supersomes were employed. Enzyme activity was assessed based on the formation of CYP-specific marker metabolites. The resulting data showed that the extract exhibited inhibitory effects on CYP2C8, CYP2C9, CYP2C19, and CYP3A4. Thus, G. pentaphyllum extract may influence the pharmacokinetics of drugs metabolized by CYP2C8, CYP2C9, CYP2C19, and CYP3A4. These findings emphasize the importance of considering potential herb-drug interactions when incorporating this extract into therapeutic regimens or dietary supplements.
Bibliography:KISTI1.1003/JNL.JAKO202331440045795
ISSN:2233-4203
2093-8950