Purification and Characterization of CDMHK, a Growth Inhibitory Molecule Against Cancer Cell Lines, from Myxobacterium sp. HK1 Isolated from Korean Soil

• Published in: Journal of microbiology and biotechnology Vol. 15; no. 4; pp. 734 - 739
• Main Authors: Han Ki Lee, In Hye Lee, Jee Sun Yim, Yong Ho Kim, Sang Hee Lee, Ki Say Lee, Yoon Mo Koo, Sang Jin Kim, Byeong Chul Jeong
• Format: Journal Article
• Language: Korean
• Published: 한국미생물생명공학회 30.08.2005
• Subjects: apoptosis; cancer cell Lines; growth inhibitor; Myxobacterium sp. HK1; apoptosis; growth inhibitor; cancer cell lines; Myxobacterium sp; HK1
• ISSN: 1017-7825; 1738-8872

Myxobacterium sp. HK1, isolated from Korean soil, degrades cellulose, differentiates to fruiting body, and its 16s rDNA has $95\%$ similarity to Polyangium sp. An anticancer molecule, CDMHK, was identified from culture broth of Myxobacterium sp. HK1, and purified by Diaion HP20, Silica gel, Sephadex LH-20 chromatography, and preparative HPLC using an YMC OSD-A C18 column. The molecular structure and formula were determined to be $C_{l2}H_{l9}N_3O_2$ (M.W 237) by MS spectrometry, 300 MHz $^{1}H\;and\;^{13}C$ NMR. The CDMHK was not active against Escherichia coli, Staphylococcus aureus, and Candida albicans. However, this molecule inhibited the growth of various cancer cell lines. The $ED_{50}$ values of CDMHK were determined to be 0.147, 0.086, 0.18, 0.166, and 0.142 $\mu$g/ml against A549, SK-OV-3, SK-MEL-2, VF498, and HCTl5 cancer cell lines, respectively. In addition, the CDMHK was able to induce apoptosis of the CCRF-CEM cancer cell line, evidenced by DNA fragmentation assay and DAPI staining.