MicroRNA-214-mediated UBC9 expression in glioma
It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9), the unique enzyme2 in the sumoylation pathway, is up-regulated in many cancers. However, the expression and regulation of UBC9 in glioma remains unknown. In this study, we found that Ubc9 was up-regulated in glioma tissues and cell lin...
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Published in | BMB reports Vol. 45; no. 11; pp. 641 - 646 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | Korean |
Published |
생화학분자생물학회
30.11.2012
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Subjects | |
Online Access | Get full text |
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Summary: | It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9), the unique enzyme2 in the sumoylation pathway, is up-regulated in many cancers. However, the expression and regulation of UBC9 in glioma remains unknown. In this study, we found that Ubc9 was up-regulated in glioma tissues and cell lines compared to a normal control. UBC9 knockdown by small interfering RNA (siRNA) affected cell proliferation and apoptosis in T98G cells. Further experiments revealed that microRNA (miR)-214 directly targeted the 3` untranslated region (UTR) of UBC9 and that there was an inverse relationship between the expression levels of miR-214 and UBC9 protein in glioma tissues and cells. MiR-214 overexpression suppressed the endogenous UBC9 protein and affected T98G cell proliferation. These findings suggest that miR-214 reduction facilitates UBC9 expression and is involved in the regulation of glioma cell proliferation. |
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Bibliography: | Korean Society for Biochemistry and Molecular Biology KISTI1.1003/JNL.JAKO201201052160025 |
ISSN: | 1976-6696 1976-670X |