MicroRNA-214-mediated UBC9 expression in glioma

It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9), the unique enzyme2 in the sumoylation pathway, is up-regulated in many cancers. However, the expression and regulation of UBC9 in glioma remains unknown. In this study, we found that Ubc9 was up-regulated in glioma tissues and cell lin...

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Published inBMB reports Vol. 45; no. 11; pp. 641 - 646
Main Authors Zhao, Zhiqiang, Tan, Xiaochao, Zhao, Ani, Zhu, Liyuan, Yin, Bin, Yuan, Jiangang, Qiang, Boqin, Peng, Xiaozhong
Format Journal Article
LanguageKorean
Published 생화학분자생물학회 30.11.2012
Subjects
Cell proliferation
Glioma
miR-214
Sumoylation
UBC9
Cell proliferation
Sumoylation
miR-214
Glioma
UBC9
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Summary:It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9), the unique enzyme2 in the sumoylation pathway, is up-regulated in many cancers. However, the expression and regulation of UBC9 in glioma remains unknown. In this study, we found that Ubc9 was up-regulated in glioma tissues and cell lines compared to a normal control. UBC9 knockdown by small interfering RNA (siRNA) affected cell proliferation and apoptosis in T98G cells. Further experiments revealed that microRNA (miR)-214 directly targeted the 3` untranslated region (UTR) of UBC9 and that there was an inverse relationship between the expression levels of miR-214 and UBC9 protein in glioma tissues and cells. MiR-214 overexpression suppressed the endogenous UBC9 protein and affected T98G cell proliferation. These findings suggest that miR-214 reduction facilitates UBC9 expression and is involved in the regulation of glioma cell proliferation.
Bibliography:Korean Society for Biochemistry and Molecular Biology
KISTI1.1003/JNL.JAKO201201052160025
ISSN:1976-6696
1976-670X