Sphingobacterium composti sp. nov., a Novel DNase-Producing Bacterium Isolated from Compost

• Published in: Journal of microbiology and biotechnology Vol. 16; no. 11; pp. 1728 - 1733
• Main Authors: Leonid N. Ten, Qing Mei Liu, Wan Taek Im, Zubair Aslam, Sung Taik Lee
• Format: Journal Article
• Language: Korean
• Published: 한국미생물생명공학회 30.11.2006
• Subjects: compost; polyphasic taxonomy; Sphingobacterium composti sp. nov; Sphingobacterium composti sp; nov; compost; polyphasic taxonomy
• ISSN: 1017-7825; 1738-8872

A Gram-negative, strictly aerobic, nonmotile, and nonspore-forming bacterial strain, designated $T5-12^T$, was isolated from compost and characterized using a polyphasic taxonomical approach. The isolate was positive for catalase and oxidase tests. It could degrade DNA, but was negative for degradation of macromolecules such as casein, collagen, starch, chitin, cellulose, and xylan. The DNA G+C content was 36.0 mol%. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The major fatty acids were $iso-C_{15:0}$ (45.6%), $iso-C_{17:0}$ 3OH (17.2%), and summed feature 4 ($C_{16:0}\;{\omega}7c$ and/or $iso-C_{15:0}$ 2OH, 14.9%). Comparative 16S rRNA gene sequence analysis showed that strain $T5-12^T$ fell within the radiation of the cluster comprising members of the genus Sphingobacterium. Strain $T5-12^T$ exhibited lower than 94% of 16S rRNA gene sequence similarity with respect to the type strains of recognized Sphingobacterium species. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain $T5-12^T$ ($=KCTC\;12578^T=LMG\;23401^T=CCUG\;52467^T$) should be classified in the genus Sphingobacterium as the type strain of a novel species, for which the name Sphingobacterium composti sp. novo is proposed.