CopA3 peptide from Copris tripartitus induces apoptosis in human Leukemia cells via a caspase-independent pathway

Our previous study demonstrated that CopA3, a disulfide dimer of the coprisin peptide analogue (LLCIALRKK), has antibacterial activity. In this study, we assessed whether CopA3 caused cellular toxicity in various mammalian cell lines. CopA3 selectively caused a marked decrease in cell viability in J...

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Published inBMB reports Vol. 45; no. 2; pp. 85 - 90
Main Authors Kang, Bo-Ram, Kim, Ho, Nam, Sung-Hee, Yun, Eun-Young, Kim, Seong-Ryul, Ahn, Mi-Young, Chang, Jong-Soo, Hwang, Jae-Sam
Format Journal Article
LanguageKorean
Published 생화학분자생물학회 29.02.2012
Subjects
Anti-cancer effect
Apoptosis
Caspase-independent apoptosis pathways
Cell membrane penetration
Insect peptide
Leukemia
Anti-cancer effect
Caspase-independent apoptosis pathways
Cell membrane penetration
Insect peptide
Leukemia
Apoptosis
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Summary:Our previous study demonstrated that CopA3, a disulfide dimer of the coprisin peptide analogue (LLCIALRKK), has antibacterial activity. In this study, we assessed whether CopA3 caused cellular toxicity in various mammalian cell lines. CopA3 selectively caused a marked decrease in cell viability in Jurkat T, U937, and AML-2 cells (human leukemia cells), but was not cytotoxic to Caki or Hela cells. Fragmentation of DNA, a marker of apoptosis, was also confirmed in the leukemia cell lines, but not in the other cells. CopA3-induced apoptosis in leukemia cells was mediated by apoptosis inducing factor (AIF), indicating induction of a caspase-independent signaling pathway. [BMB reports 2012; 45(2): 85-90].
Bibliography:Korean Society for Biochemistry and Molecular Biology
KISTI1.1003/JNL.JAKO201208636393954
ISSN:1976-6696
1976-670X