Black Rice (Oryza sativa L. var. japonica) Hydrolyzed Peptides Induce Expression of Hyaluronan Synthase 2 Gene in HaCaT Keratinocytes

Black rice (Oryza sativa L. var. japonica) has been used in folk medicine in Asia. To understand the effects of black rice hydrolyzed peptides (BRP) from germinated black rice, we assessed the expression levels of about 20,000 transcripts in BRP-treated HaCaT keratinocytes using human 1A oligo micro...

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Published inJournal of microbiology and biotechnology Vol. 17; no. 2; pp. 271 - 279
Main Authors Sim, Gwan-Sub, Lee, Dong-Hwan, Kim, Jin-Hwa, An, Sung-Kwan, Choe, Tae-Boo, Kwon, Tae-Jong, Pyo, Hyeong-Bae, Lee, Bum-Chun
Format Journal Article
LanguageKorean
Published 한국미생물생명공학회 28.02.2007
Subjects
germinated black rice
hyaluronan synthase
microarray
Oryza sativa
peptides
Oryza sativa
peptides
microarray
hyaluronan synthase
germinated black rice
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Summary:Black rice (Oryza sativa L. var. japonica) has been used in folk medicine in Asia. To understand the effects of black rice hydrolyzed peptides (BRP) from germinated black rice, we assessed the expression levels of about 20,000 transcripts in BRP-treated HaCaT keratinocytes using human 1A oligo microarray analysis. As a result, the BRP treatment showed a differential expression ratio of more than 2-fold: 745 were activated and 1,011 were repressed. One of the most interesting findings was a 2-fold increase in hyaluronan synthase 2 (HAS2) gene expression by BRP. Semiquantitative RT-PCR showed that BRP increased HAS2 mRNA in dose-dependent manners. ELISA showed that BRP effectively increased hyaluronan (HA) production in HaCaT keratinocytes.
Bibliography:The Korean Society for Applied Microbiology
KISTI1.1003/JNL.JAKO200710912610894
ISSN:1017-7825
1738-8872