Biosynthesis of Glycosylated Derivatives of Tylosin in Streptomyces venezuelae

Streptomyces venezuelae YJ028, bearing a deletion of the entire biosynthetic gene cluster encoding the pikromycin polyketide synthases and desosamine biosynthetic enzymes, was used as a bioconversion system for combinatorial biosynthesis of glycosylated derivatives of tylosin. Two engineered deoxysu...

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Published inJournal of microbiology and biotechnology Vol. 21; no. 6; pp. 613 - 616
Main Authors Han, Ah-Reum, Park, Sung-Ryeol, Park, Je-Won, Lee, Eun-Yeol, Kim, Dong-Myung, Kim, Byung-Gee, Yoon, Yeo-Joon
Format Journal Article
LanguageKorean
Published 한국미생물생명공학회 30.06.2011
Subjects
combinatorial biosynthesis
Streptomyces venezuelae
Tylosin
Streptomyces venezuelae
combinatorial biosynthesis
Tylosin
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Summary:Streptomyces venezuelae YJ028, bearing a deletion of the entire biosynthetic gene cluster encoding the pikromycin polyketide synthases and desosamine biosynthetic enzymes, was used as a bioconversion system for combinatorial biosynthesis of glycosylated derivatives of tylosin. Two engineered deoxysugar biosynthetic pathways for the biosynthesis of TDP-3-O-demethyl-D-chalcose or TDP-Lrhamnose in conjunction with the glycosyltransferaseauxiliary protein pair DesVII/DesVIII were expressed in a S. venezuelae YJ028 mutant strain. Supplementation of each mutant strain capable of producing TDP-3-O-demethyl- D-chalcose or TDP-L-rhamnose with tylosin aglycone tylactone resulted in the production of the 3-O-demethyl- D-chalcose, D-quinovose, or L-rhamnose-glycosylated tylactone.
Bibliography:The Korean Society for Applied Microbiology
KISTI1.1003/JNL.JAKO201106737197914
ISSN:1017-7825
1738-8872