NF-κB 저해를 통한 브로콜리 잎 추출물의 PGE 2 저해효과

• Published in: 大韓本草學會誌 Vol. 34; no. 6; pp. 117 - 124
• Main Authors: 박숙자, Sook Jahr Park, 안이슬, Iseul An, 노규표, Gyu Pyo Noh, 유병혁, Byung Hyuk Yoo, 이종록, Jong Rok Lee
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 30.11.2019
• Subjects: Broccoli leaf; inflammatory cytokine; NF-κB; PGE 2; Broccoli leaf; PGE_2; inflammatory cytokine; NF- ${\kappa}B
• ISSN: 1229-1765

Objective : Broccoli is edible green plant that has a wide variety of health benefits including cancer prevention and cholesterol reduction. However, leaves of broccoli are not eaten and are mostly left as waste. This study was conducted to evaluate the effects of the broccoli leaf extract (BLE) on prostaglandin E 2 (PGE 2 ) production related to nuclear factor kappa B (NF-κB) signaling in lipopolysaccharide (LPS)-activated macrophages. Methods : BLE was prepared by extracting dried leaf with ethanol. Cell viability was determined by 3-(4,5- Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. PGE 2 and inflammatory cytokines were detected by enzyme-linked immunosorbent assay (ELISA). Expression level of each protein was monitored by Western blot analysis. Results : In LPS-activated Raw264.7 cells, PGE 2 release into culture medium was dramatically enhanced compared to control cells. However, increased PGE 2 was attenuated dose-dependently by treatment with BLE. Inhibition of PGE 2 production by BLE was due to the suppression of cyclooxygenase-2 (COX-2) expression determined by Western blot analysis. BLE also inhibited the production of inflammatory cytokines such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α). Inhibition at PGE 2 and cytokine was mediated from inhibition of nuclear translocation of NF-κB due to the repression of inhibitory kappa B alpha (IκBα) phosphorylation and degradation. Conclusion : This study showed that BLE exerted inhibitory activities against PGE 2 , which is critical for the initiation and resolution of inflammatory responses, and that inhibition of PGE 2 was mediated by suppression of NF-κB signaling. These results suggest that the waste broccoli leaves could be used for controlling inflammation.