Effect of silk fibroin peptide derived from silkworm Bombyx mori on the anti-inflammatory effect of Tat-SOD in a mice edema model

• Published in: BMB reports Vol. 44; no. 12; pp. 787 - 792
• Main Authors: Kim, Dae-Won, Hwang, Hyun-Sook, Kim, Duk-Soo, Sheen, Seung-Hoon, Heo, Dong-Hwa, Hwang, Gyo-Jun, Kang, Suk-Hyung, Kweon, Hae-Yong, Jo, You-Young, Kang, Seok-Woo, Lee, Kwang-Gill, Park, Kye-Won, Han, Kyu-Hyung, Park, Jin-Seu, Eum, Won-Sik, Cho, Yong-Jun, Choi, Hyun-Chul, Choi, Soo-Young
• Format: Journal Article
• Language: Korean
• Published: 생화학분자생물학회 30.12.2011
• Subjects: Inflammation; Protein therapy; Silk fibroin; Tat-superoxide dismutase; TPA; Silk fibroin; Inflammation; TPA; Protein therapy; Tat-superoxide dismutase
• ISSN: 1976-6696; 1976-670X

We investigated whether silk fibroin peptide derived from the silkworm, Bombyx mori, could inhibit inflammation and enhance the anti-inflammatory activity of Tat-superoxide dismutase (Tat-SOD), which was previously reported to effectively penetrate various cells and tissues and exert anti-oxidative activity in a mouse model of inflammation. Inflammation was induced by topical treatment of mouse ears with 12-O-tetradecanoylphorbol- 13-acetate (TPA). Histological, Western blot, and reverse transcription-polymerase chain reaction data demonstrated that silk fibroin peptide or Tat-SOD alone could suppress elevated levels of cyclooxygenase-2, interleukin-6, interleukin-1beta, and tumor necrosis factor-alpha induced by TPA. Moreover, silk fibroin peptide significantly enhanced the anti-inflammatory activity of Tat-SOD, although it had no influence on in vitro and in vivo transduction of Tat-SOD. Silk fibroin peptide exhibited anti- inflammatory activity in a mice model of inflammation. Therefore, silk fibroin peptide alone or in combination with Tat-SOD might be used as a therapeutic agent for various inflammatory diseases. [BMB reports 2011; 44(12): 787-792]