헬리코박터 파이로리 배양에서 CO2 농도 차이에 따른 단백체 조성 비교

• Published in: Journal of bacteriology and virology Vol. 37; no. 4; pp. 213 - 224
• Main Authors: 이승규, Seung Gyu Lee, 김경미, Kyung Mi Kim, 박정원, Jeong Won Park, 권영철, Young Chul Kwon, 송재영, Jae Young Song, 배동원, Dong Won Bae, 윤희상, Hee Shang Youn, 서지현, Ji Hyun Seo, 백승철, Seung Chul Baik, 이우곤, Woo Kon Lee, 조명제, Myung Je
• Format: Journal Article
• Language: Korean
• Published: 대한바이러스학회 30.11.2007; 대한미생물학회
• Subjects: Environment stress; Helicobacter pylori; Proteome; Helicobacter pylori; Environment stress; Proteome
• ISSN: 1598-2467; 2093-0429

Helicobacter pylori is a spiral, slow growing gram-negative microaerophilic bacterium. It has been shown to be the etiological agent of gastroduodenal diseases, such as chronic gastritis, gastric and duodenal ulcers, and gastric cancer. General culture condition of H. pylori is 5% O2, l0% CO2 and 100% humid atmosphere. We have compared proliferation protein expression profile of H. pylori incubated under normal microaerophilic (l0% CO2) and environment stress (4% CO2, 18% CO2) conditions. H. pylori cultured under environment stress displayed coccoid morphology and time-dependent decrease in proliferation. We have further compared the protein expression profiles of H. pylori under normal growing and environment stress conditions by a global proteomic analysis, which includes high-resolution 2-DE followed by matrix-assisted laser desorption/ionization time of flight and nanoelectrospray/tandem mass spectrometry. In total, 42 protein spots were found to be up- or down-regulated by more than 2-fold under environment stress conditions. Of the 42 protein spots processed, 27 spots were identified; they represented 19 genes, including 2 kinds of hypothetical proteins.