Study of Cell-mediated Response in Mice by HPV16 L1 Virus-Like Particles Expressed in Saccharomyces cerevisiae

The first vaccine against human papillomaviruses (HPV) formulated with HPV16 L1 virus-like particles (VLPs) produced in yeast was approved by the FDA in June 2006. Nevertheless, there have been few studies of the immunogenicity in mice of VLPs. In this study, we evaluated the cell-mediated immune re...

Full description

Saved in:
Bibliographic Details
Published inJournal of microbiology and biotechnology Vol. 17; no. 10; pp. 1738 - 1741
Main Authors Woo, Mi-Kyung, Hur, Sook-Jin, Park, Sue-NIe, Kim, Hong-Jin
Format Journal Article
LanguageKorean
Published 한국미생물생명공학회 30.10.2007
Subjects
cytokines
Human papillomavirus
splenocytes proliferation
Th1/Th2 response
virus-Like particles
virus-like particles
Human papillomavirus
cytokines
Th1/Th2 response
splenocytes proliferation
Online AccessGet full text

Cover

More Information
Summary:The first vaccine against human papillomaviruses (HPV) formulated with HPV16 L1 virus-like particles (VLPs) produced in yeast was approved by the FDA in June 2006. Nevertheless, there have been few studies of the immunogenicity in mice of VLPs. In this study, we evaluated the cell-mediated immune response to VLPs produced in Saccharomyces cerevisiae. After immunization of mice with HPV16 L1 VLPs, we measured splenocytes proliferation and the levels of IFN$_{\gamma}$, IL2, IL4, and IL5. Splenocytes proliferation was significantly increased and a mixed Th1/Th2 response was indicated. IgG subtype immunoresponses were strongly induced and IgG1 titers were higher than those of IgG2a.
Bibliography:The Korean Society for Applied Microbiology
KISTI1.1003/JNL.JAKO200709906364943
ISSN:1017-7825
1738-8872