Protection of Radiation-Induced DNA Damage by Functional Cosmeceutical Poly-Gamma-Glutamate

This study compared the radioprotective effects of high-molecular-weight poly-gammaglutamate (γ-PGA, average molecular mass 3,000 kDa) and a reduced form of glutathione (GSH, a known radioprotector) on calf thymus DNA damage. The radiation-induced DNA damage was measured on the basis of the decrease...

Full description

Saved in:
Bibliographic Details
Published inJournal of microbiology and biotechnology Vol. 28; no. 4; pp. 527 - 533
Main Authors Oh, Yu-Jin, Kwak, Mi-Sun, Sung, Moon-Hee
Format Journal Article
LanguageKorean
Published 한국미생물생명공학회 30.04.2018
Subjects
DNA damage
DNA protection
gamma radiation
Poly-gamma-glutamate
radioprotective material
gamma radiation
radioprotective material
Poly-gamma-glutamate
DNA protection
DNA damage
Online AccessGet full text

Cover

More Information
Summary:This study compared the radioprotective effects of high-molecular-weight poly-gammaglutamate (γ-PGA, average molecular mass 3,000 kDa) and a reduced form of glutathione (GSH, a known radioprotector) on calf thymus DNA damage. The radiation-induced DNA damage was measured on the basis of the decreased fluorescence intensity after binding the DNA with ethidium bromide. All the experiments used 60 Co gamma radiation at 1,252 Gy, representing 50% DNA damage. When increasing the concentration of γ-PGA from 0.33 to 1.65 μM, the DNA protection from radiation-induced damage also increased, with a maximum of 87% protection. Meanwhile, the maximal DNA protection when increasing the concentration of GSH was only 70%. Therefore, γ-PGA exhibited significant radioprotective effects against gamma irradiation.
Bibliography:The Korean Society for Applied Microbiology
KISTI1.1003/JNL.JAKO201814442073702
ISSN:1017-7825
1738-8872