Identification and Molecular Characterization of Novel cry1-Type Toxin Genes from Bacillus thuringiensis K1 Isolated in Korea

To clone novel cry1-type genes from the Bacillus thuringiensis K1 isolate, about 2.4-kb-long PCR fragments were amplified with two primer sets of ATG1-F/N400-R and 1BeATG1-F/N400-R. Using PCR-RFLP, three novel cry1-type genes, cry1-1, cry1-7, and cry1-44, were obtained from B. thuringiensis K1 and t...

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Published inJournal of microbiology and biotechnology Vol. 17; no. 1; pp. 15 - 20
Main Authors Ming Shun Li, Jae Young Choi, Jong Yul Roh, Hee Jin Shim, Joong Nam Kang, Yang Su Kim, Yong Wang, Zi Niu Yu, Byung Rae Jin, Yeon Ho Je
Format Journal Article
LanguageKorean
Published 한국미생물생명공학회 30.01.2007
Subjects
Bacillus thuringiensis K1
baculovirus expression vector system
cry1-type gene
insecticidal activity
inverse PCR
RFLP
Bacillus thuringiensis K1
insecticidal activity
RFLP
cry1-type gene
baculovirus expression vector system
inverse PCR
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Summary:To clone novel cry1-type genes from the Bacillus thuringiensis K1 isolate, about 2.4-kb-long PCR fragments were amplified with two primer sets of ATG1-F/N400-R and 1BeATG1-F/N400-R. Using PCR-RFLP, three novel cry1-type genes, cry1-1, cry1-7, and cry1-44, were obtained from B. thuringiensis K1 and the complete coding sequences of these novel genes were analyzed. The Cry1-1, Cry1-7, and Cry1-44 proteins showed maximum similarities of about 78.0%, 99.7%, and 91.0% with the Cry1Ha1, Cry1Be1, and Cry1Ac2 proteins, respectively. These novel cry1-type genes were expressed using a baculovirus expression vector system and their insecticidal activities were investigated. Whereas all three novel genes were toxic to Plutella xylostella larvae, only Cry1-1 showed insecticidal activity against Spodoptera exigua larvae.
Bibliography:The Korean Society for Applied Microbiology
KISTI1.1003/JNL.JAKO200708506317622
ISSN:1017-7825
1738-8872