Enhanced (R)-2-(4-Hydroxyphenoxy)Propionic Acid Production by Beauveria bassiana: Optimization of Culture Medium and H 2 O 2 Supplement under Static Cultivation

• Published in: Journal of microbiology and biotechnology Vol. 30; no. 8; pp. 1252 - 1260
• Main Authors: Hu, Hai-Feng, Zhou, Hai-Yan, Wang, Xian-Lin, Wang, Yuan-Shan, Xue, Ya-Ping, Zheng, Yu-Guo
• Format: Journal Article
• Language: Korean
• Published: 한국미생물생명공학회 31.08.2020
• Subjects: 2; 4-hydroxyphenoxy; Beauveria bassiana; optimization; propionic acid; R; reactive oxygen species; static cultivation; Beauveria bassiana; optimization; static cultivation; (R)-2-(4-hydroxyphenoxy)propionic acid; reactive oxygen species
• ISSN: 1017-7825; 1738-8872

(R)-2-(4-hydroxyphenoxy)propionic acid (HPOPA) is a key intermediate for the preparation of aryloxyphenoxypropionic acid herbicides (R-isomer). In order to improve the HPOPA production from the substrate (R)-2-phenoxypropionic acid (POPA) with Beauveria bassiana CCN-A7, static cultivation and H 2 O 2 addition were attempted and found to be conducive to the task at hand. This is the first report on HPOPA production under static cultivation and reactive oxygen species (ROS) induction. On this premise, the cultivation conditions and fermentation medium compositions were optimized. As a result, the optimal carbon source, organic nitrogen source, and inorganic nitrogen source were determined to be glucose, peptone, and ammonium sulfate, respectively. The optimal inoculum size and fermentation temperature were 13.3% and 28℃, respectively. The significant factors including glucose, peptone, and H 2 O 2 , identified based on Plackett-Burman design, were further optimized through Central Composite Design (CCD). The optimal concentrations were as follows: glucose 38.81 g/l, peptone 7.28 g/l, and H 2 O 2 1.08 g/l/100 ml. Under the optimized conditions, HPOPA titer was improved from 9.60 g/l to 19.53 g/l, representing an increase of 2.03- fold. The results obtained in this work will provide novel strategies for improving the biosynthesis of hydroxy aromatics.