국내 HIV - 1 분리주에 대한 zidovudine의 저항성 연구

• Published in: Journal of bacteriology and virology Vol. 27; no. 1; pp. 77 - 86
• Main Authors: 남정구, 강춘, 이주실, 이홍래, 신동윤, 박용근, 신영오
• Format: Journal Article
• Language: Korean
• Published: 대한바이러스학회 30.06.1997
• Subjects: AZT resistance Syncytium formation HIV-1 reverse transcriptase HIV-1 p24 antigen; HIV-1 p24 antigen; HIV-1 reverse transcriptase; AZT resistance; Syncytium formation
• ISSN: 1598-2467; 2093-0429

To examine AZT resistance of HIV-1 isolates from AZT treated or untreated Korean, several biological characteristics such as syncytium formation, HIV-1 reverse transcriptase activity and the p24 antigen production in MT-2 cells infected with 4 HIV-1 isolates were determined. As controls, we tested HIV-1 HTLV-IIIB and pre-drug isolate as AZT susceptible strains, in addition to HIV-1 RTMC/MT-2 and post-drug isolate as AZT resistant strains. When the inoculum size of HIV-1 was 300 TCID /well and 100 TCID /well, the AZT susceptibility of AZT untreated HIV-1 isolates 8806 and 9571 were similar to that of HIV-1 HTLV-IIIB and AZT-susceptible HIV-1 strains. When we evaluated AZT resistance of isolates HIV-1 8812 and 9113 treated with AZT for 36 months by observation of syncytium formation, HIV-1 8812 showed resistance simillar to that of HIV-1 RTMC/MT-2 strain forming syncytium up to AZT 1pg/ml, and HIV-1 9113 showed resistance identical with that of AZT- esistant HIV-1 strain which formed syncytium up to AZT 10 pg/ml. Especially, when we evaluated AZT resistance by HIV-1 reverse transcriptase activity and the p24 antigen production, HIV-1 isolates 8812 and 9113 showed much higher resistance (>10 200 fold) compared with HIV-1 RTMC/MT-2 and AZT-resistant HIV-1 strain.