Production of Bacterial Quorum Sensing Antagonists, Caffeoyl- and Feruloyl-HSL, by an Artificial Biosynthetic Pathway

A new series comprising phenylacetyl-homoserine lactones (HSLs), caffeoyl-HSL and feruloyl-HSL, was biologically synthesized using an artificial de novo biosynthetic pathway. We developed an Escherichia coli system containing artificial biosynthetic pathways that yield phenylacetyl-HSLs from simple...

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Published inJournal of microbiology and biotechnology Vol. 27; no. 12; pp. 2104 - 2111
Main Authors Kang, Sun-Young, Kim, Bo-Min, Heo, Kyung Taek, Jang, Jae-Hyuk, Kim, Won-Gon, Hong, Young-Soo
Format Journal Article
LanguageKorean
Published 한국미생물생명공학회 31.12.2017
Subjects
Artificial biosynthesis
caffeoyl-HSL
feruloyl-HSL
phenylacetyl homoserine lactone
Artificial biosynthesis
caffeoyl-HSL
feruloyl-HSL
phenylacetyl homoserine lactone
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Summary:A new series comprising phenylacetyl-homoserine lactones (HSLs), caffeoyl-HSL and feruloyl-HSL, was biologically synthesized using an artificial de novo biosynthetic pathway. We developed an Escherichia coli system containing artificial biosynthetic pathways that yield phenylacetyl-HSLs from simple carbon sources. These artificial biosynthetic pathways contained the LuxI-type synthase gene (rpaI) in addition to caffeoyl-CoA and feruloyl-CoA biosynthetic genes, respectively. Finally, the yields for caffeoyl-HSL and feruloyl-HSL were 97.1 ± 10.3 and 65.2 ± 5.7 mg/l, respectively, by tyrosine-overproducing E. coli with a L-methionine feeding strategy. In a quorum sensing (QS) competition assay, feruloyl-HSL and p-coumaroyl-HSL antagonized the QS receptor TraR in Agrobacterium tumefaciens NT1, whereas caffeoyl-HSL did not.
Bibliography:The Korean Society for Applied Microbiology
KISTI1.1003/JNL.JAKO201708034064260
ISSN:1017-7825
1738-8872