Measurement of Iron - dependence of pupA Promoter Activity by a pup - lux Bioreporter

The promoter region of the pupA gene of Pseudomonas putida WCS358 was fused with the structural genes for bioluminescence (luxCDABE) from Vibrio fischeri, and the resulting fusion plasmid harbored by the WCS358 host. The pup-lux fusion gene was then used for quantitative analysis of the iron-depende...

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Bibliographic Details
Published inJournal of microbiology and biotechnology Vol. 7; no. 5; pp. 352 - 355
Main Authors Khang, Yong-Ho, Yang, Zamin-K, Burlage, Robert-S
Format Journal Article
LanguageKorean
Published 한국미생물생명공학회 01.10.1997
Subjects
pupA bioreporter
Fe(III)
pup-lux
siderophores
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Summary:The promoter region of the pupA gene of Pseudomonas putida WCS358 was fused with the structural genes for bioluminescence (luxCDABE) from Vibrio fischeri, and the resulting fusion plasmid harbored by the WCS358 host. The pup-lux fusion gene was then used for quantitative analysis of the iron-dependence of pupA promoter activity. Factors affecting bioluminescence produced by the pup-lux bioreporter were found to be cell activity, iron-chelator concentrations, Fe(III) concentrations, and nutrient components. Light production rates of the pup-lux bioreporter were inversely dependent upon iron molecules when $FeCl_3$ concentrations were between $10^{-2}$ and 1 ${\mu}M$ in nutrient-poor minimal media, and between 0.1 and 10 mM in nutrient-rich complex media.
Bibliography:The Korean Society for Applied Microbiology
KISTI1.1003/JNL.JAKO199711922240009
ISSN:1017-7825
1738-8872