Measurement of Iron - dependence of pupA Promoter Activity by a pup - lux Bioreporter
The promoter region of the pupA gene of Pseudomonas putida WCS358 was fused with the structural genes for bioluminescence (luxCDABE) from Vibrio fischeri, and the resulting fusion plasmid harbored by the WCS358 host. The pup-lux fusion gene was then used for quantitative analysis of the iron-depende...
Saved in:
Published in | Journal of microbiology and biotechnology Vol. 7; no. 5; pp. 352 - 355 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | Korean |
Published |
한국미생물생명공학회
01.10.1997
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The promoter region of the pupA gene of Pseudomonas putida WCS358 was fused with the structural genes for bioluminescence (luxCDABE) from Vibrio fischeri, and the resulting fusion plasmid harbored by the WCS358 host. The pup-lux fusion gene was then used for quantitative analysis of the iron-dependence of pupA promoter activity. Factors affecting bioluminescence produced by the pup-lux bioreporter were found to be cell activity, iron-chelator concentrations, Fe(III) concentrations, and nutrient components. Light production rates of the pup-lux bioreporter were inversely dependent upon iron molecules when $FeCl_3$ concentrations were between $10^{-2}$ and 1 ${\mu}M$ in nutrient-poor minimal media, and between 0.1 and 10 mM in nutrient-rich complex media. |
---|---|
Bibliography: | The Korean Society for Applied Microbiology KISTI1.1003/JNL.JAKO199711922240009 |
ISSN: | 1017-7825 1738-8872 |