Properties of the Fusants of Lactobacillus acidophilus 88 and Lactobacillus casei subsp . casei KCTC 1121

• Published in: Journal of microbiology and biotechnology Vol. 7; no. 1; pp. 25 - 31
• Main Authors: Jo, Young-Bae, Heo, Kyeong, Kim, Sung-Koo, Baik, Hyung-Suk, Jun, Hong-Ki
• Format: Journal Article
• Language: Korean
• Published: 한국미생물생명공학회 01.02.1997
• Subjects: Lactobacillus acidophilus 88; Lactobacillus casei; protoplast fusion
• ISSN: 1017-7825; 1738-8872

Protoplast fusion between L. casei KCTC 1121 and L. acidophilus 88 was attempted to obtain improved strains. The fusants produced a bacteriocin against indicator strains, making a smaller inhibition zone compared to that of L. acidophilus 88. After culturing for 2 months on selective medium, the selected fusants were still stable without segregation. Fusants showed higher lipase activity compared to those of the two parent strains. Fusant No.4, 11, and 15 exhibited excellent lactic acid productivity. Fusant No.4 and 15 exhibited improved proteolysis ability compared to the two parent strains. Whereas L. casei possessed both ${\beta}-galactosidase$ and $phospho-{\beta}-galactosidase$ activities, and L. acidophilus 88 had only ${\beta}-galactosidase$ activity, the fusants had both the intermediate enzyme activities. Cell size of the fusants was greater than that of the parents.