인슐린 비의존형 당뇨병 및 비만증 환자에서의 PPARγ2 유전자 다형성

• Published in: The Korean journal of medicine Vol. 59; no. 2; pp. 132 - 141
• Main Authors: 오승준, Seung Joon Oh, 강성이, Sung Yi Kang, 김영설, Young Seol Kim, 김덕윤, Deog Yoon Kim, 우정택, 김성운, Sung Woon Kim, 양인명, In Myung Yang, 김진우, Jin Woo Kim, 최영길, Young Kil Choi, 박혜순, He Soon Park, 팽정령, Jung Ryung Paeng
• Format: Journal Article
• Language: Korean
• Published: 대한내과학회 01.08.2000
• Subjects: non-insulin- dependent; Obesity; Hyperlipidemiai; Insulin resistance; Polymerase chain reaction; Polymorphism; Peroxisome proliferator activated receptor γ; Diabetes mellitus; Restriction fragment length
• ISSN: 1738-9364

Background : Peroxisome proliferator activated receptor-gamma (PPAR-γ) is a nuclear receptor that regulate adipocyte differentiation and modulate intracellular insulin-signaling events. As such, PPARγ is a candidate gene for several human disorders including obesity and type 2 diabetes mellitus. The objective of our study was to examine the relationship between genetic variation of PPARγ2 and diabetes and obesity in Korean subjects. Methods : We studied 99 subjects with type 2 diabetes mellitus, 128 obesity patients and 97 controls. Screening for mutation at codon 12 and 115 of PPARγ2 were carried out by PCR-RFLP analyses. Statistical significance was evaluated by Chi-square test. Results : The allele frequency of the Pro12Ala PPARγ2 variant were 0.05 in controls, 0.06 in type 2 diabetes group, and 0.07 in obesity group (p=0.47). Pro115Gln variant were only proline homozygote in all groups. Genotype frequencies were also similar and conformed to expectations of the Hardy-Weinberg rule. The presence of PPARγ2 gene variant was no associated with concentrations of total cholesterol, triglyceride, HDL-cholesterol, and also with fasting glucose. Conclusion : We concluded that the Pro12Ala and Pro115Gln PPARγ2 missense mutation may not be associated with type 2 diabetes mellitus and obesity in Korean patients.(Korean J Med 59:132-141, 2000)