Host-Controlled Cell-to-Cell Movement of a Hybrid Barley Stripe Mosaic Virus Expressing a Dianthovirus Movement Protein

• Published in: Intervirology Vol. 40; no. 1; pp. 1 - 6
• Main Authors: Solovyev, A.G., Zelenina, D.A., Savenkov, E.I., Grdzelishvili, V.Z., Morozov, S.Yu, Maiss, E., Casper, R., Atabekov, J.G.
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland S. Karger AG 01.01.1997
• Subjects: barley stripe mosaic virus; Fabaceae - virology; Hordeum - virology; Mosaic Viruses - genetics; Mosaic Viruses - pathogenicity; Mosaic Viruses - physiology; Nicotiana - virology; Original Paper; Plant Leaves - virology; Plant Viral Movement Proteins; Plants, Medicinal; Plants, Toxic; Recombinant Fusion Proteins; RNA - genetics; RNA, Viral - genetics; Viral Proteins - genetics; Viral Proteins - physiology; Cell-to-cell movement; Hybrid virus genome
• ISSN: 0300-5526; 1423-0100
• DOI: 10.1159/000150514

The triple gene block (TGB) of barley stripe mosaic virus (BSMV), coding for viral movement proteins (MPs), was replaced by the single MP gene of red clover necrotic mosaic virus (RCNMV). Accumulation of the hybrid virus in barley plants (the selective host for BSMV) was reduced compared to BSMV. The hybrid virus induced small necrotic local lesions on Chenopodium amaranticolor leaves and did not infect Nicotiana clevelandii (te selective host for RCNMV). The hybrid virus accumulated in the inoculated leaves of Nicotiana benthamiana, but not in the upper noninoculated leaves. Thus the RCNMV MP gene substituted for the BSMV TGB in cell-to-cell movement, but not in systemic spread. Hybrid virus movement was efficient only in N. benthamiana, the common host for BSMV and RCNMV. These data point to the involvement of host-specific factors in the function of virus-coded transport determinants.