혈관내피세포에서 산양삼 추출물과 진세노사이드 Rg5의 혈관신생 효과

• Published in: Han'guk Sikp'um Kwahakhoe Chi = Korean Journal of Food Science and Technology Vol. 50; no. 3; pp. 349 - 355
• Main Authors: 김나은(Na-Eun Kim), 이미옥(Mi-Ok Lee), 장미희(Mi-Hee Jang), 정병희(Byung-Hee Chung)
• Format: Journal Article
• Language: Korean
• Published: Seoul 한국식품과학회 01.06.2018; Korean Society of Food Science & Technology
• Subjects: AKT protein; Angiogenesis; Cancer; Diabetes mellitus; Endothelial cells; Extracellular signal-regulated kinase; Ginseng; Ginsenosides; Hypertension; Inflammation; Inflammatory response; Intercellular adhesion molecule 1; Molecular modelling; Nitric oxide; Phosphorylation; Umbilical vein; Vascular cell adhesion molecule 1; 식품과학; ginsenoside Rg5; signal cascade; wood-cultivated ginseng; angiogenesis; vascular endothelial growth factor
• ISSN: 0367-6293; 2383-9635
• DOI: 10.9721/KJFST.2018.50.3.349

Ginsenoside Rg5, one of the protopanaxadiol ginsenosides of wood-cultivated ginseng, has been implicated in various diseases, such as diabetes, cancer, and hypertension; however, its angiogenic activity and molecular mechanisms have not yet been elucidated. Here, we found that wood-cultivated ginseng extract and ginsenoside Rg5 increase in vitro proliferation, migration, and tube-like structure formation, which are typical phenomena associated with angiogenesis, in cultured human umbilical vein endothelial cells (HUVECs). Moreover, Ginsenoside Rg5 stimulated the phosphorylation of Akt, endothelial nitric oxide (NO) synthase (eNOS), and extracellular-regulated kinase (ERK)1/2, which are well-known signal mediators of the angiogenic pathway. Furthermore, Ginsenoside Rg5 did not accelerate the activation of ICAM-1 and VCAM-1 which are inflammatory response mediators. These results suggest that wood-cultivated ginseng extract and ginsenoside Rg5 stimulated in vitro angiogenesis by activating the Akt/eNOS- and ERK1/2-dependent signal pathways without inducing vascular inflammation.