국내산 초피와 산초의 산업적 활용 가능성: 고당으로 유도된 뇌신경세포 독성에 대한 추출물의 보호 효과

• Published in: Han'guk Sikp'um Kwahakhoe Chi = Korean Journal of Food Science and Technology Vol. 52; no. 3; pp. 274 - 283
• Main Authors: 한혜주(Hye Ju Han), 박선경(Seon Kyeong Park), 김민지(Min Ji Kim), 안준우(Jun Woo An), 이세진(Se Jin Lee), 강진용(Jin Yong Kang), 김종민(Jong Min Kim), 허호진(Ho Jin Heo)
• Format: Journal Article
• Language: Korean
• Published: Seoul 한국식품과학회 01.06.2020; Korean Society of Food Science & Technology
• Subjects: Advanced glycosylation end products; Antioxidants; Caffeoylquinic acid; Carbohydrates; Cytotoxicity; Diabetes; Diabetes mellitus; Glucose; Glucosidase; Glycosylation; Malondialdehyde; Neuroprotection; Neurotoxicity; Phenolic compounds; Phenols; Physiological effects; Physiology; Quercetin; Scavenging; Toxicity; Zanthoxylum; Zanthoxylum schinifolium; α-Amylase; α-Glucosidase; 식품과학; Zanthoxylum schinifolium; Zanthoxylum piperitum; high glucose; diabetes; neuroprotection
• ISSN: 0367-6293; 2383-9635
• DOI: 10.9721/KJFST.2020.52.3.274

This study focused on the in vitro investigation of antioxidant and anti-diabetic activities, along with neuroprotection against high glucose-induced cytotoxicity, in order to evaluate the physiological effects of Zanthoxylum piperitum and Zanthoxylum schinifolium. The highest total phenolic content was measured in the 40% ethanolic extracts of Zanthoxylum piperitum (EZP) and Zanthoxylum schinifolium (EZS). The in vitro EZP antioxidant activity showed a relatively higher ABTS/DPPH radical scavenging activity and malondialdehyde inhibitory effect than that of EZS. The EZP inhibited carbohydrate hydrolysis (α-glucosidase and α-amylase) more efficiently than EZS in anti-diabetic tests. However, EZS showed a more efficient inhibition of advanced glycation end-products formation than EZP. In addition, both EZP and EZS effectively protected human-derived neuronal cells from high glucose-induced cytotoxicity. Finally, the physiological compounds were analyzed using UPLC IMS-QTOF/MSE , and the main EZP (quercetin-3-O-glucoside and 3caffeoylquinic acid) and EZS (5-caffeoylquinic acid) compounds were identified as phenolic compounds.