발효산삼배양근농축액의 산화방지 효과 및 과산화수소로 유발된 PC12 세포독성 보호효과

• Published in: Han'guk Sikp'um Kwahakhoe Chi = Korean Journal of Food Science and Technology Vol. 50; no. 4; pp. 383 - 390
• Main Authors: 최여옥(Yeo Ok Choi), 김유리(Yu-Ri Kim), 신승용(Seung-Yong Shin), 이재근(Jae Geun Lee), 김철중(Chul Joong Kim), 이예지(Ye ji Lee), 강병주(Byeongju Kang), 김관수(Gwansu Kim), 최지은(Jee Eun Choi), 한범석(Beom-Seok Han)
• Format: Journal Article
• Language: Korean
• Published: Seoul 한국식품과학회 01.08.2018; Korean Society of Food Science & Technology
• Subjects: Cell culture; Dilution; Fermented food; Ginseng; Ginsenosides; Hydrogen peroxide; Inhibition; Iron sulfates; Lipid peroxidation; Lipids; Neurodegenerative diseases; Neurological diseases; Neuroprotection; Oxidative stress; Peroxidation; Pheochromocytoma cells; Reactive oxygen species; Scavenging; 식품과학; PC12 cell; neurodegenerative diseases; ginsenoside Rg5; ginsenoside Rk1; cultivated wild ginseng; oxidative stress
• ISSN: 0367-6293; 2383-9635
• DOI: 10.9721/KJFST.2018.50.4.383

Most neurodegenerative diseases are known to be influenced by oxidative stress. We investigated the antioxidative activity of the concentrate of fermented wild ginseng root culture (HLJG0701) containing ginsenosides Rg5 and Rk1. HLJG0701 showed effective DPPH and ABTS radical scavenging ability (IC50: 16- and 4-fold dilution, respectively) and was inhibited dose-dependently by the FeSO4-induced lipid peroxidation group (8- and 4-fold dilution: 2.3 and 1.5 nM, respectively). In MTT and LDH assays, 8-, 16-, 32- and 64-fold diluted HLJG0701 significantly increased cell viability by 70, 53, 35, and 26%, respectively. LDH released by HLJG0701 was reduced 1.3-fold with 8-fold diluted HLJG0701 compared to the H2O2-treated control. In addition, the inhibitory effect of HLJG0701 on oxidative stress in PC12 cells was confirmed by DCF-DA analysis (16-, 4-fold diluted HLJG0701: 50 and 68% ROS inhibition, respectively), TBARS (16- and 4-fold diluted HLJG0701: 50.7 and 46.5% inhibition, respectively), GPx (16- and 4-fold diluted HLJG0701: 133.3 and 227.3% release, respectively), and SOD analysis (16- and 4-fold diluted HLJG0701: 118.2 and 218.2% release, respectively). These results suggested that HLJG0701 protects neuronal cells by its anti-oxidative effects and hence can be a potential preventive material against neurodegenerative diseases.