단풍취 열수 추출물의 HepG2 인간간세포의 LPS/D-Gal에 의해 유발된 급성 간 손상에 대한 간보호 효과

• Published in: Han'guk Sikp'um Kwahakhoe Chi = Korean Journal of Food Science and Technology Vol. 52; no. 5; pp. 476 - 481
• Main Authors: 이선엽(Sun-Yeop Lee), 한준희(Joon-Hee Han), 최다혜(Da-Hye Choi), 홍민(Min Hong ), 권태형(Tae-Hyung Kwon), 이용진(Yong-Jin Lee), 유근형(Keun-Hyung Yu)
• Format: Journal Article
• Language: Korean
• Published: Seoul 한국식품과학회 01.10.2020; Korean Society of Food Science & Technology
• Subjects: Acids; Antioxidants; Aspartate transaminase; Caffeoylquinic acid; D-Galactosamine; Hepatotoxicity; L-Lactate dehydrogenase; Lactate dehydrogenase; Lactic acid; Lipopolysaccharides; Liver; Scavenging; Secretion; Sulfonic acid; Transaminase; Tumor necrosis factor-TNF; Tumor necrosis factor-α; 식품과학; Ainsliaea acerifolia; LPS/D-GalN; water extract; hepatoprotective effect
• ISSN: 0367-6293; 2383-9635
• DOI: 10.9721/KJFST.2020.52.5.476

This study investigated the antioxidant and hepatoprotective effects of Ainsliaea acerifolia water extract (AAWE) on HepG2 cells. Five types of caffeoylquinic acid (CQA) were detected in AAWE, namely, 4,5-di-O-caffeoylquinic acid (4,5-DCQA; 11.16 mg/g), 3,4-di-O-caffeoylquinic acid (3,4-DCQA; 5.23 mg/g), 5-O-caffeoylquinic acid (5-CQA; 4.88 mg/ g), 3,5-di-O-caffeoylquinic acid (3,5-DCQA; 3.51 mg/g), and 4-O-caffeoylquinic acid (4-CQA; 3.31 mg/g). AAWE exerted ABTS+ antioxidant effects, evidenced by polyphenol content and 2,2'2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH radical scavenging) activities. AAWE (300 µg/mL) treatment significantly decreased the activities of gamma glutamyl transferase (GGT), aspartate transaminase (AST), and lactate dehydrogenase (LDH) as compared to control and exerted protective effects against the increase in liver function index induced by lipopolysaccharide (LPS)/galactosamine (D-GalN) in HepG2 cells. In addition, the secretion of tumor necrosis factor (TNF)-α by HepG2 cells induced by LPS/D-GalN significantly increased in all treatment groups compared to that in the control. However, AAWE (100-300 µg/mL) treatment significantly decreased the secretion of TNF-α compared to that in the control. These results suggest that AAWE treatment reduces hepatotoxicity by increasing antioxidant activities, reducing GGT, AST, and LDH activities, and inhibiting TNF-α secretion.