보리발효추출물로부터 분리한 다당의 대식세포 활성화 및 신호 전달
Barley has nutritional benefits due to its high dietary fiber content; therefore, the intake of whole barley grains is recommended. However, barley is often consumed in the fermented form because of the improved texture and digestibility. The present study was designed to elucidate the intracellular...
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Published in | Han'guk Sikp'um Kwahakhoe Chi = Korean Journal of Food Science and Technology Vol. 50; no. 5; pp. 555 - 563 |
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Main Authors | , , |
Format | Journal Article |
Language | Korean |
Published |
Seoul
한국식품과학회
01.10.2018
Korean Society of Food Science & Technology |
Subjects | |
Online Access | Get full text |
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Summary: | Barley has nutritional benefits due to its high dietary fiber content; therefore, the intake of whole barley grains is recommended. However, barley is often consumed in the fermented form because of the improved texture and digestibility. The present study was designed to elucidate the intracellular signaling pathway for macrophage activation by the polysaccharide BF-CP from fermented barley. BF-CP is a neutral polysaccharide, composed of neutral sugars, including glucose (70.7%), xylose (11.4%), and arabinose (9.0%). BF-CP exhibited macrophage-stimulatory activity by inducing the production of interleukin (IL)-6, tumor necrosis factor (TNF)-α, and nitric oxide in RAW 264.7 macrophages. Further, BFCP treatment strongly increased the IL-6 and TNF-α gene expression in a concentration-dependent manner. Signal transduction experiments using immunoblotting showed that BF-CP phosphorylated mitogen-activated protein kinases (MAPKs), such as c-Jun N-terminal kinase, extracellular signal-regulated kinase, and p38, and nuclear factor (NF)-κB, in RAW 264.7 cells in a concentration-dependent manner. These results suggest that BF-CP activates the macrophages via MAPK and NF-κB pathways, and also induces an increase in the production of cytokines. |
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Bibliography: | KISTI1.1003/JNL.JAKO201833469090678 |
ISSN: | 0367-6293 2383-9635 |
DOI: | 10.9721/KJFST.2018.50.5.555 |