옥돔과 옥두어 판별을 위한 PCR 검사법 개발과 검증

• Published in: Han'guk Sikp'um Kwahakhoe Chi = Korean Journal of Food Science and Technology Vol. 51; no. 3; pp. 295 - 299
• Main Authors: 김나예슬(Na-Ye-Seul Kim), 양지영(Ji-Young Yang), 김중범(Jung-Beom Kim)
• Format: Journal Article
• Language: Korean
• Published: Seoul 한국식품과학회 01.06.2019; Korean Society of Food Science & Technology
• Subjects: Branchiostegus albus; Branchiostegus japonicus; Cytochrome; Cytochrome b; Cytochromes; Multiplexing; Primers; 식품과학; monitoring; Branchiostegus albus; PCR; validation; Branchiostegus japonicus
• ISSN: 0367-6293; 2383-9635
• DOI: 10.9721/KJFST.2019.51.3.295

We developed and validated species-specific primers for Branchiostegus japonicus and Branchiostegus albus to prevent the sale of B. albus as B. japonicus. Primers for B. japonicus and B. albus were designed against the cytochrome b gene. Multiplex PCR showed a 288 bp amplicon for B. japonicus, a 159 bp amplicon for B. albus, and a 502 bp amplicon for the internal control. The PCR product bands for B. japonicus, B. albus, and the internal control were present at 1 ng each. The specificity and sensitivity of the primers developed in this study were validated by testing 38 B. japonicus strains and 13 B. albus strains. Using this monitoring method, fake fish did not appear due to the agreement between the experimental results and the species. Therefore, the developed multiplex PCR method was suitable for differentiating B. japonicus and B. albus.