혈관내피세포에서 꽃송이버섯(Sparassis crispa) 소수성 추출물의 항혈관신생 활성

• Published in: Han'guk Sikp'um Kwahakhoe Chi = Korean Journal of Food Science and Technology Vol. 51; no. 2; pp. 141 - 146
• Main Authors: 한장미(Jang Mi Han), 공소연(So Youn Gong), 송재경(Jae Kyung Sohng), 강예재(Yue Jai Kang), 정혜진(Hye Jin Jung)
• Format: Journal Article
• Language: Korean
• Published: Seoul 한국식품과학회 01.04.2019; Korean Society of Food Science & Technology
• Subjects: AKT protein; Angiogenesis; Antiangiogenics; Anticancer properties; Biocompatibility; Chloroform; Chorioallantoic membrane; Cytotoxicity; Diabetes mellitus; Embryos; Endothelial cells; Growth factors; Inflammation; Invasiveness; Mushrooms; Phosphorylation; Sparassis crispa; Toxicity; Umbilical vein; Vascular endothelial growth factor; 식품과학; Sparassis crispa; non-aqueous fraction; VEGFR2 signaling; angiogenesis; HUVECs
• ISSN: 0367-6293; 2383-9635
• DOI: 10.9721/KJFST.2019.51.2.141

Sparassis crispa is an edible mushroom that is distributed in Korea, Japan, Europe, and North America. It exerts various biological activities such as immunopotentiation, anti-diabetic, anti-cancer, and anti-inflammatory effects. Recently, we separated the health functional non-aqueous fraction from the chloroform extract of S. crispa (SCF4). In this study, we evaluated the antiangiogenic activity of SCF4 in human umbilical vein endothelial cells (HUVECs). SCF4 effectively inhibited vascular endothelial growth factor (VEGF)-induced cell growth at concentrations (5-25 µg/mL) showing no cytotoxic effects. SCF4 inhibited VEGF-induced invasiveness and tube formation ability, which are in vitro angiogenic features of HUVECs, in a dose-dependent manner. In addition, SCF4 markedly suppressed in vivo angiogenesis of chorioallantoic membrane from growing chick embryos without cytotoxicity. Furthermore, SCF4 downregulated the phosphorylation of VEGFR2, AKT, and ERK1/2, which are major angiogenic signal mediators. These results suggest that SCF4 inhibited angiogenesis by suppressing the VEGFR2 signaling pathways without cytotoxicity.