Single Clone per PCR에 의한 HIV-1 env 유전자의 Quasispecies 분석

• Published in: Journal of bacteriology and virology Vol. 35; no. 2; pp. 133 - 140
• Main Authors: 양한나, Han Na Yang, 남정구, Jeong Gu Nam, 김성순, Sung Soon Kim, 최상윤, Sang Yun Choi, 이주실, Joo Shil Lee
• Format: Journal Article
• Language: Korean
• Published: 대한바이러스학회 2005; 대한미생물학회
• Subjects: env gene; HIV; Molecular cloning; Quasispecies; 생물학; Quasispecies; env gene; HIV; Molecular cloning
• ISSN: 1598-2467; 2093-0429

The human immunodeficiency viruses (HIV) exhibit tremendous genetic variability in their hosts. It is mainly due to two factors: the error-prone nature of the viral reverse transcriptase enzyme and the effects of environmental constraints such as antiviral therapy, cellular tropism, or HIV-specific immune responses. These quasispecies show fluctuation both in the overall divergence and diversity between individual sequences with different duration after primary infection. For better understand the viral quasispecies, we have performed the longitudinal genetic analysis of HIV-1 env gene V1-C5 region (1.2 kb) by two molecular cloning methods. Diversity indicated that 'single clone per PCR' value was higher than that of 'multiple clones per PCR' in subjects; 0.58-3.15 in subject 1 (P<0.05) and 0.28-2.25 in subject 2 (P<0.05). But divergence was similar in both molecular cloning methods. Phylogeneitc analysis of longitudinal sequences at different sampling stages revealed the existence of different topologies individually. These data suggested that 'single clone per PCR' is more efficient than 'multiple clones per PCR' in genetic diversity analysis.