콩 배아로 부터 Isoflavone과 Soyasaponin의 동시 분리

• Published in: Korean journal of crop science Vol. 58; no. 2; pp. 149 - 160
• Main Authors: 김선림, Sun-lim Kim, 이재은, Jae-eun Lee, 김율호, Yul-ho Kim, 정건호, Gun-ho Jung, 김대욱, Dea-wook Kim, 이춘기, Choon-ki Lee, 김미정, Mi-jung Kim, 김정태, Jung-tae Kim, 이유영, Yu-young Lee, 황태영, Tae-young Hwang, 이광식, Kwang-sik Lee, 김욱한, Wook-han Kim, 권영업, Young-up Kwon, 김홍식, Hong-sig Kim, 정일민, Ill-min Chung
• Format: Journal Article
• Language: Korean
• Published: 한국작물학회 30.06.2013
• Subjects: isoflavone; isolation; soy germ; soyasaponin; soybean; 농학; soybean; isoflavone; isolation; soy germ; soyasaponin
• ISSN: 0252-9777; 2287-8432
• DOI: 10.7740/kjcs.2013.58.2.149

The objective of present study was to simultaneously isolate of isoflavone and soyasaponin compounds from the germ of soybean seeds. Soy germ flours were defatted with hexane for 48h at room temperature, and methanolic extracts were prepared using reflux apparatus at 90℃ for 6h, two times. After extraction, extracts were separated with preparative RP-C18 packing column (125 Å, 55-105 μm, 40×150mm), and collected 52 fractions were identified with TLC plate (Kieselgel 60 F-254) and HPLC, respectively. Among the identified isoflavone and soyasaponin fractions, isoflavone fractions were re-separated using a recycling HPLC with gel permeation column (Jaigel-W252, 20×500mm). Final fractions were air-dried, and the purified compounds of two isoflavones (ISF-1-1, ISF-1-2) and four soyasaponins (SAP-1, SAP-2, SAP-3, SAP-4) were obtained. Two isoflavone compounds (ISF-1-1, ISF-1-2) were acid-hydrolyzed for the identification of their aglycones, and confirmed by comparing with 12 types of isoflavone isomers. While the four kinds of soyasaponins were identified by using a micro Q-TOF mass spectrometer in the ESI positive mode with capillary voltage of 4.5kV, and dry temperature of 200℃. Base on the obtained results, it was conclude that ISF-1-1 is the mixture isomers of daidzin (43.4%), glycitin (47.0%), and genistin (9.6%), but ISF-1-2 is the single compound of genistin (99.8% <). On the other hand, soyasaponin SAP-1 is the mixture compounds of soyasaponin A-group (Aa, Ab, Ac, Ae, Af); SAP-2 is soyasaponin B-group (Ba, Bb, Bc) and E-group (Bd, Be); SAP-3 is soyasaponin B-group (Ba, Bb, Bc), E-group (Bd, Be), and DDMP-group (βg); SAP-4 is soyasaponin B-group (Ba, Bb, Bc), E-group (Bd, Be), and DDMP-group (βg, βa), respectively.